Development of biomimetic sensors to determine the functionality of in vitro cell models

Authors

  • MATJAŽ FRANGEŽ University of Maribor, Faculty of Medicine, Maribor, Slovenia , Univerza Maribor, Medicinska Fakulteta, Maribor, Slovenija https://orcid.org/0009-0001-9750-1826 (unauthenticated)
  • NOAH EMIL GLISIK University of Maribor, Faculty of Medicine, Maribor, Slovenia , Univerza Maribor, Medicinska Fakulteta, Maribor, Slovenija https://orcid.org/0009-0000-5567-0472 (unauthenticated)
  • MAŠA KAISER University of Maribor, Faculty of Medicine, Maribor, Slovenia , Univerza Maribor, Medicinska Fakulteta, Maribor, Slovenija
  • JARO VEZJAK University of Maribor, Faculty of Medicine, Maribor, Slovenia , Univerza Maribor, Medicinska Fakulteta, Maribor, Slovenija
  • JURE TERDIN University of Maribor, Faculty of Medicine, Maribor, Slovenia , Univerza Maribor, Medicinska Fakulteta, Maribor, Slovenija
  • MATIC ŽNIDARŠIČ University of Maribor, Faculty of Medicine, Maribor, Slovenia , Univerza Maribor, Medicinska Fakulteta, Maribor, Slovenija
  • MATIC ŽNIDARŠIČ University of Maribor, Faculty of Medicine, Maribor, Slovenia , Univerza Maribor, Medicinska Fakulteta, Maribor, Slovenija
  • TANJA ZIDARIČ University of Maribor, Faculty of Medicine, Maribor, Slovenia , Univerza Maribor, Medicinska Fakulteta, Maribor, Slovenija https://orcid.org/0000-0002-5466-510X (unauthenticated)
  • TINA MAVER University of Maribor, Faculty of Medicine, Maribor, Slovenia , Univerza Maribor, Medicinska Fakulteta, Maribor, Slovenija https://orcid.org/0000-0002-1876-7248 (unauthenticated)

DOI:

https://doi.org/10.18690/analipazu.14.2.39-57.2024

Keywords:

biomimetic sensors, molecular imprinting, polypyrrole matrix, insulin, MIP sensor

Abstract

Advances in biomedical science have enabled the development of in vitro models that mimic human tissues. Non-invasive real-time monitoring of these models would provide valuable insights without disrupting the cellular environment. This study explores the use of molecularly imprinted polymers (MIPs) for this purpose, focusing on insulin and lactate as biomarkers of cellular metabolism. MIP sensors were created by electropolymerizing pyrrole around insulin or lactate templates on carbon electrodes. The sensors showed high sensitivity and selectivity, with detection ranges of 20.0–70.0 pM for insulin (LOD: 2.41 pM) and 0.5–3.0 mM for lactate. These findings highlight MIPs’ potential for personalized diagnostics and therapy monitoring.

Author Biographies

  • MATJAŽ FRANGEŽ, University of Maribor, Faculty of Medicine, Maribor, Slovenia, Univerza Maribor, Medicinska Fakulteta, Maribor, Slovenija

    MATJAŽ FRANGEŽ

    University of Maribor, Faculty of Medicine, Maribor, Slovenia

    E-mail: matjaz.frangez1@student.um.si

  • NOAH EMIL GLISIK, University of Maribor, Faculty of Medicine, Maribor, Slovenia, Univerza Maribor, Medicinska Fakulteta, Maribor, Slovenija

    NOAH EMIL GLISIK

    University of Maribor, Faculty of Medicine, Maribor, Slovenia

    E-mail: noah.glisik@student.um.si

     

  • MAŠA KAISER, University of Maribor, Faculty of Medicine, Maribor, Slovenia, Univerza Maribor, Medicinska Fakulteta, Maribor, Slovenija

    MAŠA KAISER

    University of Maribor, Faculty of Medicine, Maribor, Slovenia

    E-mail: masa.kaiser@student.um.si

     

  • JARO VEZJAK, University of Maribor, Faculty of Medicine, Maribor, Slovenia, Univerza Maribor, Medicinska Fakulteta, Maribor, Slovenija

    JARO VEZJAK

    University of Maribor, Faculty of Medicine, Maribor, Slovenia

    E-mail: jaro.vezjak@student.um.si

  • JURE TERDIN, University of Maribor, Faculty of Medicine, Maribor, Slovenia, Univerza Maribor, Medicinska Fakulteta, Maribor, Slovenija

    JURE TERDIN

    University of Maribor, Faculty of Medicine, Maribor, Slovenia

    E-mail: jure.terdin@student.um.si

  • MATIC ŽNIDARŠIČ, University of Maribor, Faculty of Medicine, Maribor, Slovenia, Univerza Maribor, Medicinska Fakulteta, Maribor, Slovenija

    MATIC ŽNIDARŠIČ

    University of Maribor, Faculty of Medicine, Maribor, Slovenia

    E-mail: matic.znidarsic@student.um.si

  • MATIC ŽNIDARŠIČ, University of Maribor, Faculty of Medicine, Maribor, Slovenia, Univerza Maribor, Medicinska Fakulteta, Maribor, Slovenija

    MATIC ŽNIDARŠIČ

    University of Maribor, Faculty of Medicine, Maribor, Slovenia

    E-mail: matic.znidarsic@student.um.si

  • TANJA ZIDARIČ, University of Maribor, Faculty of Medicine, Maribor, Slovenia, Univerza Maribor, Medicinska Fakulteta, Maribor, Slovenija

    TANJA ZIDARIČ

    University of Maribor, Faculty of Medicine, Maribor, Slovenia

    E-mail: tanja.zidaric@um.si

  • TINA MAVER, University of Maribor, Faculty of Medicine, Maribor, Slovenia, Univerza Maribor, Medicinska Fakulteta, Maribor, Slovenija

    TINA MAVERž

    University of Maribor, Faculty of Medicine, Maribor, Slovenia

    E-mail: tina.maver@um.si

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Published

25.12.2024

Issue

Vol. 14 No. 2 (2024): ANALI PAZU

Section

Prispevki

How to Cite

FRANGEŽ, M., GLISIK, N. E., KAISER, M., VEZJAK, J., TERDIN, J., ŽNIDARŠIČ, M., ŽNIDARŠIČ, M., ZIDARIČ, T., & MAVER, T. (2024). Development of biomimetic sensors to determine the functionality of in vitro cell models. Anali PAZU, 14(2), 39-57. https://doi.org/10.18690/analipazu.14.2.39-57.2024

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